A TECHNIQUE--INSTRUCTIONS HOW TO CLONE A HUMAN: The ovulation and ovaries of the woman can be monitored. Just before natural ovulation, there is an increase of luteinizing hormone which is called the luteinizing hormone surge. This can be detected by either blood or urine samples. The growth of the follicle can be monitored by visualization with ovarian ultrasonography. Ultrasound diagnosis will reveal on which side of the woman’s ovaries the ripening follicle is found. This procedure will allow people to know when the follicle is ripe for the retrieval of the oocyte. When the time is appropriate a hollow aspiration needle is inserted into one or several ripe follicles under visual guidance of the laparoscope. The oocyte is removed with some follicular fluid. Experienced laparoscopists have a success rate over 90% in recovering the oocyte. Prior to this, it is likely that the woman will have been given Clomiphene citrate, or this drug used in combination with another drug so that there will be several eggs that can be retrieved at one time. The oocytes obtained from the ripe ovarian follicles are not fertilized when retrieved, although another process would be to fertilize first, before extracting. If they don’t fertilize first, then they can take the harvested oocytes and incubate them in a culture medium for several hours to get maturation. They need maturation because they have been taken from the ovary before ovulation, and are not as mature as spontaneously ovulated ova. Thawed or fresh semen is washed and certifuged so that it will be diluted to the proper concentration to fertilize in vitro. The in vitro fertilization is carried out. After some amount of hours, (about 12) both pronuclei are identifiable for enucleation. The enucleation is accomplished with either one of two well-established methods. One method is to surgically enucleate it with a micropipette, another is with a bleb of cytoplasm containing both the male and female pronuclei. Either method has worked fine. These nuclei by the way have been obtained from the inner-cell mass of an early human embryo. This again is a well established practice. Let us digress slightly and explain the method to obtain the nuclei. The zona pellucida must be removed from a cultured embryo, the trophectoderm separated from the inner cell mass, and then, the cells dissociated with an appropriate enzyme in a calcium-and magnesium-free salt solution. Going back to the cloning process, there are several methods for doing a donor nucleus (obtained from its source using the just mentioned method) with enucleated cytoplasm (obtained from the woman’s in vitro fertilized ovum). One might be to surgically implant it with a micropipette, another is fusion with an inactivated Sendai virus. Whichever way is considered most viable by those performing this will be used. And then the human nuclear transplant will be cultured until it can be placed into a human foster mother. When the clone has reached the 8 - to 16 cell stage it will be transferred into the foster mother. If needed, the transfer can be done later, and the clone is simply frozen. When the transfer takes place, the clone is drawn into a fine plastic tube (a catheter) which, then in turn, would be introduced through the cervical canal into the interior of the uterus. --- This is just one process for successfully cloning humans. Other more refined techniques may well be in use.