Easy-nlc 1200 Getting Started Guide



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Thermo
EASY-nLC 1200
Getting Started Guide
Touch-screen application version 4.0 60053-97272 Revision A September 2015


© 2015 Thermo Fisher Scientific Inc. All rights reserved.
AFC, EASY-nLC, EASY-Spray, EASY-column, IFC, Nanospray Flex, nanoViper, Viper, and PepMap are trademarks, and Acclaim, Dionex, LCQ Fleet, LXQ, LTQ, TSQ, and Xcalibur are registered trademarks of
Thermo Fisher Scientific Inc. in the United States.
PEEK is a trademark of Victrex PLC. PEEKsil is a trademark of SGE International Pty Ltd Corp. The following are registered trademarks in the United States Advion and RePlay are registered trademarks of
Advion Biosystems, Inc. Agilent and Varian are registered trademarks of Agilent Technologies Inc. Bruker is a registered trademark of Bruker Corporation. CMOSens is a registered trademark of Sensirion AG Corp. Duran is a registered trademark of Schott AG. IDEX is a registered trademark of IDEX Corporation. Linux is a registered trademark of Linus Torvald. Micromass and Waters are registered trademarks of Waters Corporation. PhotoMOS is a registered trademark of Panasonic Electric Works, Co, Ltd. Sciex is a registered trademark of Applied Biosystems Life Technologies. SSH is a registered trademark of Tectia Corporation. Teflon is a registered trademark of E.I. du Pont de Nemours and Company. VICI and Valco are registered trademarks of Valco Instruments Co. Inc.
Microsoft and Windows are registered trademarks of the Microsoft Corporation in the United States and other countries. All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries.
Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the product operation. This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.
The contents of this document are subject to change without notice. All technical information in this document is for reference purposes only. System configurations and specifications in this document supersede all previous information received by the purchaser.
This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This document shall in noway governor modify any Terms and Conditions of Sale, which Terms and Conditions of Sale shall govern all conflicting information between the two documents.
Release history Revision A, September Hardware versions EASY-nLC 1200 instrument
Software version Touchscreen software version 4.0


For research use only. not for use in diagnostic procedures.
Fcc compliance statement
Notice on the proper use of
Important this recycling program is not
Weee konformität
Installing the easy-nlc instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35
Contentsviii
Integrated instrument control software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51
Configuring the easy-nlc system. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69
Chapter 5preparing the easy-nlc instrument for use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .83
Contentsthermo scientificeasy-nlc 1200 getting started guideix
Chapter 6running samples using the integrated software. . . . . . . . . . . . . . . . . . . . . . . . .123
Chapter 7installing and controlling external devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . .155
To view the product manuals on the data system computer
Warning:sharp needle and moving parts.
Note highlights information of general interest.tip
Contacting us
To find product information
Introductionautosampler tray compartmentthermo scientificeasy-nlc 1200 getting started guide3
Autosampler tray compartment
Introductionback panelthermo scientificeasy-nlc 1200 getting started guide5
Elementdescription
Introductioncolumn setup optionsthermo scientificeasy-nlc 1200 getting started guide7
Two-column setup
Introductioncolumn setup optionsthermo scientificeasy-nlc 1200 getting started guide9
Table 3. syringe pump functionscomponent
Six-port rotary valves
Figure 9. check valve connections mixing tee
Overview of a sample run
Introductionpredefined steps for sample runsthermo scientificeasy-nlc 1200 getting started guide17
Refill pumps ab, and s step
Introductionpredefined steps for sample runsthermo scientificeasy-nlc 1200 getting started guide19
Introductionpredefined steps for sample runsthermo scientificeasy-nlc 1200 getting started guide21
Introductionpredefined steps for sample runsthermo scientificeasy-nlc 1200 getting started guide23
Introductionpredefined steps for sample runsthermo scientificeasy-nlc 1200 getting started guide25
Introductionpredefined steps for sample runsthermo scientificeasy-nlc 1200 getting started guide27
W1 42 63 5a
Introductionpump flow controlthermo scientificeasy-nlc 1200 getting started guide31
Figure 22. schematic diagram of the afc system 42 63 5b
Figure 23. schematic diagram of the ifc system 42 63 5a
Load speed protection system
Installing the easy-nlc instrument
Benchtop dimensions and weight capacity
Table 6. easy-nlc instrument dimensionsdimension
Easy - nlc ii
Temperature and humidity
Power inmech. pumps/accessory
To connect the easy-nlc instrument to line power
Figure 28. two-way contact closure connection for an orbitrap fusion mass spectrometer
To connect the contact closure cable
Setting up the ethernet connections to the data system computer
To connect the data system computer to the intranet
To connect the easy-nlc to the local network that has internet capability
Attaching add-on devices through the p-bus and rs ports
Integrated instrument control software
Entering alphanumeric text
To open the easy-spray (direct control) dialog box
Table 8. system readbacksreadback
Figure 39. menu and page structurequeue
Pagefunction
To start using or configuring the easy-nlc instrument’s built-in software
Figure 41. confirmation box from pressing exit in the lower left corner of the touchscreen. press logout
Configuring the easy-nlc system
To setup the mass spectrometer connection
Table 9. user types and permission levels for each menumenu
To create anew user account
Protection (lsp)
Table 10. hplc parameter descriptions (sheet 2 of 2)parameter
To check the solvent levels
Preparing the solvent bottles and waste containers
Preparing the easy-nlc instrument for use
Preparing the solvents
Table 11. solventssolvent/reagent
To install the waste beaker and prepare and install the wash bottles
To purge and flush pumps a and b
Setting up the column assembly for the nanospray flex source
Setting up a standard column assembly
Table 12. columns and column fittingsitem
Descriptionpart number
Tools parts and materials
Caution danger electric shock hazard!
Figure 73. column assembly mounted on directjunction360
To connect an emitter to the peek union
Setting up the column assembly for the easy-spray source
To connect an easy-spray column fora one-column setup to the easy-nlc 1200
Tip when you install the one-column assembly, make sure that the one column setup
Using nanoviper fittings
Equilibrating the precolumn
Itemmaximum pressure (in bar)
To prepare the sample vials or microplates
Creating a method
To start the method setup wizard
Setting up the sample pickup and loading steps
To setup the sample loading parameters
Running samples using the integrated software
To build a gradient
Table 17. custom wash parametersparameter
Viewing the batch list and setting up a batch record
Specifying the method, sample positions, and number of injections
Figure 100. start queue message box. press accept
Using the overview page in the home menu
Installing and controlling external devices
To connect the replay device to the easy-nlc instrument
Index 166



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