[ Care and Use ManUal ]

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[ Care and Use ManUal Oasis HLB Disks
I. IntroductIon
HLB is a Hydrophilic-Lipophilic-Balanced, water-wettable, reversed-phase sorbent for all your SPE needs. It is made from a specific ratio of two monomers, the hydrophilic N-vinylpyrrolidone and the lipophilic divinylbenzene. It provides superior reversed-phase capacity with a neutral polar hook for enhanced retention of polar analytes.
Waters has built a family of SPE sorbents which inherit some key features of this unique substrate stability at pH extremes and in a wide range of solvents, extraordinary retention of polar compounds, and a relative hydrophobic retention capacity 3 X higher than that of traditional silica-based SPE sorbents like C
Water-wettable Oasis sorbents exhibit excellent retention capacity fora wider polarity spectrum of analytes, even if the sorbent bed runs dry during conditioning or sample loading. This means that your
SPE methods will be more rugged and robust, obviating the need for repeat preparation.
The advantage of having higher retention capacity k is that more analytes are retained with less breakthrough improving the recovery and overall reproducibility of your SPE method.
Available in a 47 mm diameter disk format and employing 30 m particle size sorbent, Oasis HLB Disks extend your ability to perform a wide variety of complex sample separations.
I. IntroductIon
II. sample pre-treatment III. solId phase extractIon for acIdIc, neutral, and
BasIc compounds usIng oasIs hlB Iv. oasIs hlB protocol charts v. trouBleshootIng oasIs hlB dIsKs

[ Care and Use ManUal Oasis HLB Disks
II. Sample pre-TreaTmenT a. Solid Samples Soil. Homogenize the sample with an appropriate solvent to obtain an aqueous based or an organic solvent based extract of the sample. Initial extraction conditions are chosen to maximize analyte recovery, while minimizing matrix interference. In many cases, it maybe beneficial to add buffers, dispersive salts, or co-solvents to improve extraction efficiency. Adjust the initial extract to optimize analyte retention onto the SPE sorbent. This can include pH adjustment, solvent adjustment, or solvent exchange through evaporation and reconstitution (refer to Sections IV and V. It maybe necessary to centrifuge or filter the sample prior to loading.
b. Aqueous Samples Water, Beverages
Adjust pH to maximize analyte retention on the SPE sorbent. Buffer salts and dispersive agents maybe used to increase partitioning onto the SPE sorbent. Pretreatment to remove suspended matter prior to SPE treatment may include filtration or centrifugation. c. Non-Aqueous Liquid
When appropriate the sample maybe diluted with aqueous buffers and organic co-solvents for reversed-phased or mix-mode SPE. If sufficient dilution has occurred, the sample maybe treated in a manner similar to an aqueous sample. III. SolId phaSe exTracTIon for acIdIc, neuTral, and
BaSIc compoundS uSIng oaSIS hlB
(Note: refer to Table 1 for the recommended volume to be used in each step)
1. Place Oasis HLB cartridge or plate on the vacuum manifold. Condition Draw through methanol. Equilibrate: Draw through water. Load Draw through diluted sample. Wash Draw through 5% methanol in water (v/v). (Release vacuum and discard waste fluids. Insert collection device, replace the cover, and turn on vacuum. Elute: Draw through methanol (or other elution solvents, collecting eluates in a suitable device. Evaporate/Reconstitute or Dilute (optional. Inject Cover collection plate and inject onto column.
Note: For the Load and Elute steps, it is recommended that flow rates should
not exceed 1.0 mL/min In all other steps, flow rates up to 5 mL/min are
Iv. oaSIS hlB proTocol charTS
i. Generic Method Oasis HLB SPE Avoid using methods developed for Cor other silica-based cartidges see note below)
Prepare sample
(typically acidfy)
4a: Condition
4c: Load sample solution
4d: Wash methanol in water
4e: Elute
Evaporate and reconstitute
4b: Equilibrate

[ Care and Use ManUal Oasis HLB Disks
• The fast Oasis HLB generic method is ideal for LC/MS/MS analysis
• The generic method is an excellent starting point since it works fora wide range of compounds, yielding high recoveries
(> 85%) and consistent results (< 5% RSD)
• When cleaner backgrounds are required for higher sensitivity or selectivity, the generic protocol can be optimized using a straighforward method development strategy.
The strategy uses the full pH range (pH 1 to pH 14) and varies the organic solvent level.
Note: Wash and Elute steps developed for C
or other silica-based sorbents
may not be appropriate for the polymeric Oasis HLB sorbent.
ii. Strategy for Optimizing the Generic SPE Method
Prepare sample solution either in acid or base
Load sample solution
Wash 1 5% methanol in water
Basic Compounds
Acidic Compounds
Wash 2 2% formic acid in methanol/water
5% NH
OH in methanol/water
Wash 2 5% NH
OH in methanol/water
2% formic acid in methanol/water
The strategic procedure to obtain cleaner extracts start with the generic method through the first wash.
By adjusting the pH of the additional washes to increase analyte retention, higher concentrations of organic solvent maybe applied to remove interferences.
• The pH is decreased for acidic analytes (below the pKa of the compound) to increase retention The pH is raised for basic compounds (above the pKa of the compound) to increase retention The pH is then changed to elute the analyte. The % solvent in the Wash 2 and Elute steps is determined by varying the
% methanol in 10% increments at each pH.
• Analyze the Wash 2 and Elute samples to determine optimum
% methanol.
• Select the highest % methanol in Wash 2 that does not remove any analytes.
• Select the lowest % methanol in the Elute step that elutes the analytes.
Figure 1: Retention Factor Versus pH For Acids, Bases, and Neutrals
With the generic SPE method, one method yields good results fora wide range of compounds. With an optimized method, cleaner extracts can be obtained.

[ Care and Use ManUal Oasis HLB Disks
v. TrouBleShooTIng a. adjustment to optimize recoveries
Spike an appropriate volume of reagent water with all analytes and internal/surrogate standards. Follow steps 4a-4e in Section II, but use a rack to collect the eluates in the Load (c, Wash (d, and Elute (e) steps in separate collection vessels. In addition, repeat step e with a second portion of methanol and collect the eluate. Analyze all four collected fractions. Use the provided table to determine adjustments, if necessary, to optimize sample recovery.

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